Methods and Devices for Cryopreservation of Biological Cells and Tissues
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منابع مشابه
P-28: Hydrostatic Pressure Induced Cell Deathin Cumulus Cells and Improved In vitro Maturationof Oocytes from Preovulatory Follicles
Background: Cryopreservation of ovaries is an important technique in assisted reproduction technology. Physical forces like hydrostatic pressure have a pivotal role in reproduction systems. Due to changes in intrafollicular pressure during ovulatory process, this study designed to examine the effects of hydrostatic pressure on oocyte maturation and cell death in cumulus cells from cryopreserved...
متن کاملO-21: Differential Expression and Epigenetic Pattern of HOX Family Genes in Cumulus Cells of Mature MII Oocytes from Patients with Polycystic Ovary Syndrome
Background Ovarian tissue cryopreservation represents a promising strategy to preserve the ovarian function in cancer patients. It is usually performed by slow freezing/rapid thawing (SF/RT). Recent studies emphasize an ultrarapid cryopreservation procedure, vitrification/warming (V/W), since it might prevent damages due to ice crystal formation. Comparative studies between the cryopreservation...
متن کاملO-12: Clinical Grade Isolation and Cryopreservation of Human Testicular Cells
Background: More than a decade work in preservation of male fertility by spermatogonial stem cell (SSC) transplantation in animal models as well as new discoveries about the nature of human SSCs has prompted an investigation into the possibility of an effective clinicalgrade procedure for isolation and cryopreservation of human testicular cells. Materials and Methods: Clinical-grade reagents, v...
متن کاملInm-2: New Approaches in Cryopreservation
Cryopreservation of embryos and gametes has become an indispensible service for infertility treatment. Vitrification is now a prevalent technique for cryopreservation in assisted reproductive technology (ART) replacing, in many cases, conventional slow cooling methodology. Fertility preservation in female patients has become a very topical issue. Various malignant and nonmalignant diseases have...
متن کاملکرایوپرزرویشن سلول های اپی تلیال آمنیون انسانی در محیط فاقد سرم
Introduction & Objective: One of the important issues in long term storage of cells is removal of animal serum from cell culture environments. The aim of this study was to evaluate amniotic fluid (AF), which is full of growth factors, as substitute for fetal bovine serum (FBS) in the cryopreservation protocol. Materials & Methods: In this experimental study human amniotic epithelial cells wer...
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تاریخ انتشار 2017